Abstract

    This study aimed to determine whether mesenchymal stem cells (MSCs) derived from umbilical cord blood (UCB) would promote cutaneous wound healing. MSCs from human UCB were isolated and identified. The characteristics of the isolated MSCs' growth and proliferation were assayed in vitro. The MSCs labeled with 5-bromodeoxyuridine (BrdU) were applied on fresh cutaneous mice wounds. The healing rates were surveyed. The distribution and the differentiation into keratinocytes of the labeled MSCs in the wound tissue were checked by immunohistochemistry staining. The isolated MSCs could grow and proliferate well in vitro. The isolated MSCs from UCB could be labeled by 5-bromodeoxyuridine successfully. The MSCs derived from UCB could enhance the healing of mice skin defect wounds, and it was found that the implanted MSCs could differentiate into keratinocyte in the wound tissue. It was demonstrated that MSCs from UCB can be isolated and proliferated successfully. The local administration of MSCs derived from UCB improves skin defect wound healing in mice.

    本实验的目的在于了解脐血源间充质干细胞是否可以修复皮肤损伤。脐血源间充质干细胞被分离、鉴定，并于体外分析其生长及增殖情况。标记后的间充质干细胞（使用5-溴去氧尿苷BrdU）应用于小鼠新鲜皮肤伤口。我们对愈合率进行监测，并使用免疫组织化学染色的方法测定受伤组织中MSCs的迁移和分化为角质细胞的程度。在体外MSCs生长及增殖情况良好，并且使用BrdU成功标记。通过在受伤组织中发现分化为角质细胞的MSCs，证明脐血源MSCs促进了小鼠皮肤受伤组织的修复，因此也证明了MSCs可以被成功分离及增殖。本文说明局部应用脐血源间充质干细胞可以促进小鼠皮肤受损组织的修复。